In a new application note Waters describes a clinical research method using liquid-liquid extraction from Capitainer®B remote blood collection devices to analyze four immunosuppressive drugs on Waters Xevo TQ Absolute Mass Spectrometer.

Concluded benefits from the development are:

• Analytical sensitivity from low volume samples using the Xevo TQ Absolute
• Fast analytical run time, with simultaneous analysis of four analytes, afforded by the analytical selectivity of the chromatography and mass detection
• Small sample volume with a Capitainer®B device provided good precision and accuracy

Simultaneous analysis of the immunosuppressant drugs cyclosporine, everolimus, sirolimus and tacrolimus is descibed and can be achieved in a single analysis of a little over two minutes (injection-to-injection). The method shows excellent analytical sensitivity, total precision, and repeatability (≤7.6% RSD) across all analytes and concentrations tested.

This development of immunosuppressive drugs monitoring on Waters Xevo TQ Absolute adds to the evidence of the usability of the Capitainer®B device for immunosuppressive drug monitoring which also preciously has been published by Dprez et al. which also included analysis of creatinine in the LC-MS method.

We recommend our blog post An Introduction to Therapeutic Drug Monitoring and Possibilities With DBS if you want to get a background to the possibilities of TDM with quantitative DBS and Capitainer devices.

Learn more by the full application note on Waters webiste, or read and download a PDF version below.